Characterization of plant oils on a monolithic silica column by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

摘要

Five plant oils (peanut, pumpkin seed, sesame seed, soybean, and wheat germ) have been analyzed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). Gradient elution was performed with acetone-acetonitrile mobile phases on a short monolithic silica column (SilicaROD, RP-18e, 50 mm x 4.6 mm). Identification of plant oil triacylglycerols (TAG) was based on the pseudomolecular ion [M + H](+) and the diacylglycerol [M - RCO2](+) fragments. Positional isomers of triacylglycerols were identified from the relative intensities of the [M - RCO2](+) fragments. Principal-component analysis, used to find similarities and differences between the different oils, indicated that the different plant oils could be clearly differentiated according to their triacylglycerol composition. [References: 44]