The Involvement of Protein Kinase C in the Effect of Oxidized Glutathione and Glutoxim on Na~+Transport in Frog Skin

摘要

The amphibian skin and other isolated epithelialsystems serve as classic model objects to study transe-pithelial ion transport mechanisms. Na~+transport inepithelial cells is known to be a complex multicompo-nent system containing various Na~+transporting pro-teins, which may be targets for oxidative stress. Previ-ously, we have demonstrated that Na~+ transport in frogskin can be modulated by various oxidizing agents. Itwas shown for the first time that oxidized glutathione(GSSG) and its pharmacological analogue glutoximapplied to the basolateral surface of the frog skin imi-tated the effect of insulin and increased transepithelialNa~+ transport. Furthermore, we elucidated for the firsttime the involvement of tyrosine kinases and phosphati-dylinositol kinases in the stimulatory effect of GSSGand glutoxim on Na~+transport in frog skin.