Genetic Polymorphism of the Bovine MHC DRB3 Exon 2 of Umblachery Cattle Distinguished by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)*

摘要

BoLA DEB3 exon 2 region of MHC class II gene of Umblachery cattle was amplified by Hemi-nesting PCR using primers namely HL030 (forward) and HL031 (reverse) for the first round and HL030 (forward) and HL032 (reverse) forsecond round. The digestion of second round PCR products with three restriction enzymes namely Rsal, Haelll, and BsfYl resulted in seven, five and three restriction patterns respectively. It is concluded that exon 2 of BoLA-DRB3 gene of Umblachery cattleis highly polymorphic to support their disease resistance.