An enzyme-based reversible CNOT logic gate realized in a flow system?

摘要

An enzyme system organized in a flow device was used to mimic a reversible ControlledNOT (CNOT) gatewith two input and two output signals. Reversible conversion of NAD+ and NADH cofactors was used to perform a XOR logic operation, while biocatalytic hydrolysis of p-nitrophenyl phosphate resulted in an Identity operation working in parallel. The first biomolecular realization of a CNOT gate is promising for integration into complex biomolecular networks and future biosensor/biomedical applications.