ENZYMATIC RESOLUTION OF Z-GAMMA,GAMMA'-DI-TERT-BUTYL-D,L-CARBOXYGLUTAMIC ACID METHYL ESTER

摘要

A rapid and simple method for the resolution of Z-gamma,gamma'-di-tert-butyl-D,L-carboxyglutamic acid methyl eater is described. The new procedure is based on the enzymatic enantioselective saponification of the methyl eater by the endoprotease papain. Using a simple HPLC protocol the enantiomeric excess or Z-di-tert-butyl-L-Gla-OH has been shown to be higher than 99.5%. The procedures described in this communication allow the resolution, analysis, and downstream process of multi-gram amounts of the Gla derivative in 48 h. [References: 13]