EFFECTS OF TARAXACUMMONGOLICUM EXTRACT ON LIPOPOLYSACCHARIDE-INDUCEDNITRIC OXIDE AND CYTOKINES PRODUCTION BY BOVINE PERIPHERAL BLOOD MONONUCLEAR CELLS

摘要

The objective of this study is to investigate Taraxacum mongolicum (TM) as a therapeutic alternative for preventing and treating bovine mastitis. The effect of the anti-inflammatory activity of Taraxacum mongolicum extract (TME) on lipopolysaccharide(LPS)-induced responses was studied in the bovine peripheral blood mononuclear cells (PBMCs). The dried plant TM was extracted with 10 volumes of distilled water to generate its water extract. PBMCs were pretreated with various concentrations of TME (0,1, 10, 100, 1000 /Ug/mL) and subsequently incubated with LPS (1 /mug/mL). Cell viability was analyzed by 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenylthiazolium bromide (MTT) assay. The level of nitric oxide (NO) was determined by using Griess reagent assay. The mRNA expression levels of pro-inflammatory cytokines including interleukin (IL)-l/3, IL-6, IL-8, tumor necrosis factor (TNF)-q and granulocyte chemotactic protein (GCP)-2 were determined by using quantitative reverse transcription-polymerase chainreaction (qRT-PCR). The results showed no significant cytotoxic effects on the PBMCs at various treated concentrations of TME. Treatment of TME (100 and 1000 /ug/mL) significantly inhibited NO production in LPS-stimulated PBMCs. TME (10 ug/mL) significantly inhibited LPS-stimulated IL-1/3, IL-6, IL-8, TNF-a and GCP-2 mRNA expression in PBMCs at a time-dependent manner. In this article, we reported for the first time