Application of ultrasound in preparing pathological sections to reduce processing time

摘要

It has been found experimentally that application of sub-mega and low megahertz ultrasound ( US) of spatial and temporal averaged intensity I-sata up to 10 W/cm(2) during the process of preparing pathological sections of the mouse tissue has shortened the processing time from 12 h ( without US) to less than half an hour ( with US). The experiment has also showed that the processing time reached the shortest for ultrasound f = 200 kHz among the frequencies of 200 kHz, 400 kHz, 600 kHz, 800 kHz and 1 MHz used in this study. It has been proposed that ultrasound inducing non-inertial cavitation enhanced the permeability of cell membrane to liquid. Thus tissue fixation and dehydration were speeded up by application of US. (c) 2007 Elsevier B.V. All rights reserved.