Antennal and Behavioral Responses of Cis boleti to Fungal Odor of Trametes gibbosa

摘要

Cis boletf(Coleoptera: Ciidae)preferentially colonizes fungi from the genus Trametes that are known as important wood decomposers.The aim of our research was to investigate if C boleti uses the chemical volatile composition of its fungal host,Trametes gibbosa,as a key attraction factor.Therefore,the T gibbosa fruiting body volatiles were analysed by using gas chromatography-mass spectrometry,with parallel electroantennographic detection(GC-MS/EAD)using adults of C boleti.Furthermore,we examined the behavioral responses of C boleti to the T gibbosa volatile compounds.The dominant component of the T gibbosa fruiting body bouquet was 1 -octen-3-ol.Other volatiles,like the aldehydes hexanal,nonanai,and(E,E)-2,4-decadienal and the terpene alpha-bisabolol,were present in minor quantities.1 -Octen-3-ol was released with a ratio of the(R)- and(S)-enantiomers of 93:7,respectively.Electroantennography(EAG)employing C boleti antennae yielded consistently dominant responses to 1 -octen-3-ol.GC-EAD and EAG responses to pure standard compounds showed that C boleti also perceived other host fungal volatiles.A highly significant attraction to 1-octen-3-ol was observed in behavioral tests.Female beetles were significantly attracted to the(S)-(+)- enantiomer at 10 times lower doses than male beetles.Our finding is the first direct proof that did beetles use 1-octen-3-ol as a key cue for host finding.