Cloning, characterization and overexpression of a 14-3-3 omega protein from oil palm (Elaeis guineensis)

摘要

Plant 14-3-3 proteins are involved in signal transduction pathways of nitrogen and carbohydrate metabolism. An Eg14-3-3 gene was isolated from the mesocarp of oil palm. The 1055-bp cDNA had an open reading frame of 774bp that encoded for 258 amino acids, and the cDNA had 113-bp and 195-bp 5- and 3-untranslated regions, respectively. The calculated molecular weight was 28.06kDa, with a pI of 5.04. The palm 14-3-3 showed closest identity to 14-3-3 proteins of the omega group. The entire sequence of Eg14-3-3 showed 83% identity with 14-3-3 protein isoform 16R from Solanum tuberosum. Phylogenetic analysis showed that the Eg14-3-3 isoform was within the omega () subgroup and, thus, was designated Eg14-3-3 . The Eg14-3-3 expression patterns were strong in the mesocarp as compared to the root. When Eg14-3-3 cDNA was overexpressed in transgenic calli, there was higher accumulation of oil in the transgenic calli than in the controls. Therefore, Eg14-3-3 has potential for applications in the breeding of oil palms in the future.