Screening of core simple sequence repeat primer pairs and establishment of a multiplex polymerase chain reaction system for Brassica genomes A and C.

摘要

Brassica species are important industrial materials. Recently, simple sequence repeat (SSR) markers have become preferred molecular markers because of their simplicity, high levels of polymorphism and codominant nature. Numerous SSR markers have been developed in Brassica crops, but not every SSR marker has an equal value, and they must be tested individually for each application. To develop a set of standardized, highly efficient core SSR primers, we screened 190 primer pairs from among over 2353 SSR primer pairs that have been mapped for Brassica species. Ten different primer pairs were selected for each of the 19 linkage groups that corresponded with the chromosomes of Brassica genomes A and C. We also established 19 multiplex polymerase chain reaction (PCR) combinations, one per linkage group. Twenty accessions from six Brassica species were used to test these primer combinations, and an average of 15 clear bands were amplified successfully in each reaction. It is clear that multiplex PCR is a practical method for germplasm identification, genetic diversity analysis and backcross breeding in Brassica crops.Digital Object Identifier http://dx.doi.org/10.1111/j.1439-0523.2012.01955.x