Identification, inheritance and gene mapping of resistance to a virulent Soybean mosaic virus strain SC15 in soybean.

摘要

Soybean mosaic virus (SMV) is one of the major pathogens for soybeans worldwide. Among the 21 strains identified under uniform identification system in China, SC15 can infect all 10 of the differentials, is the most virulent strain and has been prevalent especially in Southern China. In this study, 10 elite accessions resistant to SC15 were identified from 205 soybean lines. P₁, P₂, F₁, F₂ and 200 recombinant inbred lines (RIL) of the cross 'RN-9' (resistant, R) x '7605' (susceptible, S) were used to study inheritance of resistance to SC15. F₁ was identical to 'RN-9', F₂ segregated in a 3R : 1S phenotypic ratio, whereas RILs segregated in a 1R : 1S genotypic ratio. This indicated that a single dominant gene, designated as R_SC15, conferred the resistance. A genetic linkage map of a segment of C2 linkage group was constructed using SSR markers identified by bulked segregant analysis of RIL population, where R_SC15 was mapped between Sat_213 and Sat_286 with distances of 8.0 and 6.6 cM to the respective flanking markers.Digital Object Identifier http://dx.doi.org/10.1111/j.1439-0523.2010.01797.x