首页> 外文期刊>Plant Breeding >Development of a sequence-specific PCR marker linked to the Ku gene which removes the vernalization requirement in narrow-leafed lupin.
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Development of a sequence-specific PCR marker linked to the Ku gene which removes the vernalization requirement in narrow-leafed lupin.

机译:与Ku基因连锁的序列特异性PCR标记的开发消除了窄叶羽扇豆的春化要求。

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摘要

Wild types of Lupinus angustifolius require vernalization to promote flowering. Modern domesticated cultivars carry the early-flowering gene Ku which removes this requirement. A microsatellite-anchored fragment length polymorphism marker was identified as co-segregating with the Ku gene in a recombinant inbred line (RIL) population derived from a domesticated x wild-type cross. DNA sequencing showed that the marker contained a 7 bp insertion/deletion polymorphism, as well as a single nucleotide polymorphism. A pair of sequence-specific primers was designed and successfully converted the size polymorphism into a simple polymerase chain reaction based co-dominant marker. This marker is closely linked to the Ku gene, as it co-segregates with the Ku phenotyping in a population consisting of 106 RILs..
机译:野生型羽扇豆需要春化以促进开花。现代驯化品种带有早开花基因Ku,从而消除了这一需求。微卫星锚定的片段长度多态性标记被标识为与Ku基因在从一个驯化的x野生型杂交衍生的重组近交系(RIL)群体中共分离。 DNA测序表明该标记物具有7 bp的插入/缺失多态性,以及单核苷酸多态性。设计了一对序列特异性引物,并将大小多态性成功转化为基于简单聚合酶链反应的共显性标记。该标记与Ku基因紧密相连,因为它与Ku表型共分离,包含106个RIL。

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