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A three-dimensional movie of structural changes in bacteriorhodopsin

机译：细菌视紫红质结构变化的三维电影

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摘要

Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.
机译：细菌视紫红质（bR）是光驱动的质子泵和模型膜转运蛋白。我们在x射线自由电子激光下使用时间分辨的系列飞秒晶体学来观察光激活后bR的构象变化从纳秒到毫秒。最初扭曲的视网膜生色团在蛋白质的细胞质侧置换了跨膜螺旋F的保守色氨酸残基，而在细胞外侧置换了关键的水分子。整个蛋白质的结构变化级联反应表明，当bR将质子逆着跨膜浓度梯度向上运输时，如何编排运动。

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  • 来源
    《Science》 |2016年第6319期|1552-1557|共6页
  • 作者

    Nango Eriko; Royant Antoine; Kubo Minoru; Nakane Takanori; Wickstrand Cecilia; Kimura Tetsunari; Tanaka Tomoyuki; Tono Kensuke; Song Changyong; Tanaka Rie; Arima Toshi; Yamashita Ayumi; Kobayashi Jun; Hosaka Toshiaki; Mizohata Eiichi; Nogly Przemyslaw; Sugahara Michihiro; Nam Daewoong; Nomura Takashi; Shimamura Tatsuro; Im Dohyun; Fujiwara Takaaki; Yamanaka Yasuaki; Jeon Byeonghyun; Nishizawa Tomohiro; Oda Kazumasa; Fukuda Masahiro; Andersson Rebecka; Bath Petra; Dods Robert; Davidsson Jan; Matsuoka Shigeru; Kawatake Satoshi; Murata Michio; Nureki Osamu; Owada Shigeki; Kameshima Takashi; Hatsui Takaki; Joti Yasumasa; Schertler Gebhard; Yabashi Makina; Bondar Ana-Nicoleta; Standfuss Jorg; Neutze Richard; Iwata So;

  • 作者单位

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan|Kyoto Univ, Dept Cell Biol, Grad Sch Med, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan;

    Univ Grenoble Alpes, CNRS, Commissariat Energie Atom & Energies Alternat, Inst Biol Struct, F-38044 Grenoble, France|European Synchrotron Radiat Facil, F-38043 Grenoble, France;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan|Japan Sci & Technol Agcy JST, Precursory Res Embryon Sci & Technol PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan;

    Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan;

    Univ Gothenburg, Dept Chem & Mol Biol, Box 462, SE-40530 Gothenburg, Sweden;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan|Kobe Univ, Grad Sch Sci, Dept Chem, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan|Pohang Univ Sci & Technol, Dept Phys, Pohang 790784, South Korea;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    RIKEN Ctr Life Sci Technol, Div Struct & Synthet Biol, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan;

    Osaka Univ, Grad Sch Engn, Dept Appl Chem, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan;

    Paul Scherrer Inst, Lab Biomol Res, Div Biol & Chem, CH-5232 Villigen, Switzerland;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    Pohang Univ Sci & Technol, Dept Phys, Pohang 790784, South Korea;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    Kyoto Univ, Dept Cell Biol, Grad Sch Med, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan;

    Kyoto Univ, Dept Cell Biol, Grad Sch Med, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan;

    Kyoto Univ, Dept Cell Biol, Grad Sch Med, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan;

    Kyoto Univ, Dept Cell Biol, Grad Sch Med, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan;

    Pohang Univ Sci & Technol, Dept Phys, Pohang 790784, South Korea;

    Japan Sci & Technol Agcy JST, Precursory Res Embryon Sci & Technol PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan|Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan;

    Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan;

    Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan;

    Univ Gothenburg, Dept Chem & Mol Biol, Box 462, SE-40530 Gothenburg, Sweden;

    Univ Gothenburg, Dept Chem & Mol Biol, Box 462, SE-40530 Gothenburg, Sweden;

    Univ Gothenburg, Dept Chem & Mol Biol, Box 462, SE-40530 Gothenburg, Sweden;

    Uppsala Univ, Dept Chem, Angstrom Lab, Uppsala, Sweden;

    Osaka Univ, Lipid Act Struct Project, JST Exploratory Res Adv Technol ERATO, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan;

    Osaka Univ, Lipid Act Struct Project, JST Exploratory Res Adv Technol ERATO, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan;

    Osaka Univ, Lipid Act Struct Project, JST Exploratory Res Adv Technol ERATO, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan;

    Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan;

    Paul Scherrer Inst, Lab Biomol Res, Div Biol & Chem, CH-5232 Villigen, Switzerland|Swiss Fed Inst Technol, Dept Biol, CH-8093 Zurich, Switzerland;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;

    Free Univ Berlin, Dept Phys, Theoret Mol Biophys, Arnimallee 14, D-14195 Berlin, Germany;

    Paul Scherrer Inst, Lab Biomol Res, Div Biol & Chem, CH-5232 Villigen, Switzerland;

    Univ Gothenburg, Dept Chem & Mol Biol, Box 462, SE-40530 Gothenburg, Sweden;

    RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan|Kyoto Univ, Dept Cell Biol, Grad Sch Med, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan;

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