Quantum Dot-Based Western Blot Technology for Ultrasensitive Detection of Tracer Proteins

摘要

The application notes of Quantum Dot Corporation (USA),published in the January 2005 issue of Nature Methods,undoubtedly demonstrate the potential of quantum dot (QD)-based immuno-blotting technology in multiplex detection of proteins or protein states from a single western blot.The described procedure takes the same time as standard western blot analysis with a simple colorimetric detection.The QD-labeled blots can be stored in a buffer at 4-8 deg C with minimal loss of signal for imaging at a later date.Moreover,the narrow emission of QD-labeled protein blots enables simplified image acquisition and quantification that give a possibility to overcome the semiquantitative nature of western blot analysis.