Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells

摘要

The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analysed. The changes which are not directly linked to melanogenesis in the B-1 6 / C3 cultures during their maturation were characterized. Early (1 day), confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine amino-peptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.