EVALUATION METHOD FOR ACTIVITY OF MESENCHYMAL STEM CELLS, CULTURE METHOD FOR MESENCHYMAL STEM CELLS, PRODUCTION METHOD OF THERAPEUTIC AGENT FOR IMPAIRED LIVER FUNCTIONS, AND THERAPEUTIC AGENT FOR IMPAIRED LIVER FUNCTIONS

摘要

SOLUTION: To provide an evaluation method for evaluating the degree of activity of mesenchymal stem cells, to provide a culture method for mesenchymal stem cells using the evaluation method thereof, to provide a production method of a therapeutic agent for impaired liver functions, and to provide a therapeutic agent for impaired liver functions, in a field of culturing mesenchymal stem cells for regenerative medicine, and to provide an activity evaluation method for mesenchymal stem cells according to the invention comprising a measurement step of measuring the amount of adenylate kinase 4 (AK4) in mesenchymal stem cells and a determination step of determining the degree of activity of the mesenchymal stem cells from the amount of AK4 measured.EFFECT: The degree of activity of mesenchymal stem cells can be evaluated from the amount of AK4 measured. For example, if the degree of mitochondrial activity of mesenchymal stem cells is high, it can be evaluated that the mesenchymal stem cells are suitable for use as a therapeutic agent, on the other hand, if the mitochondrial activity of mesenchymal stem cells is low and aging is not progressing, it can be evaluated that the mesenchymal stem cells are suitable for subculture.SELECTED DRAWING: Figure 1