Effect of New Immunosuppressant-Rapamycin on One-way Mixed Lymphocyte Culture

摘要

Objective: Observing human to mouse one-way mixed lymphocyte culture(xMLC) and the effect of new immunosuppressant-Rapamycin on XMLC. Methods: Mouse splenic lymphocyte were collected and treated by mit-omycin as activating cell; Human Peripheral blood lymphocytes(hPBL) were separated and gathered as reacting cell; Mouse splenic lymphocyte and hPBL wee mixed to incubate for 1 week; The researchers designed control RPM groups, and experiment(drugs)grup have different concentration. Results: HPBL in the experiment groups (mixed mouse lymphocyte) proliferated obviously, the amount of ~3H-TdR in corporation increased evidently(P < 0.05,The mean percentage of CD_4, CD_8, LgG, LgM positive cells rose markedly. HPBL in the experiment groups less proliferated, the amount of ~3H-TdR incorporation declined, RPM's ic50(50% inhibition concentration)approximately in 1.5 nmol/L; the mean percentage of CD_4 , CD_8, IgG, IgM positive cells fell obviously. Conclusion: The human to mouse one-way MLC has obvious lymphocyte proliferation. New immunosuppressants-Rapamycin have powerful effete on XMLC.