A novel method for screening of potential probiotics for high adhesion capability

摘要

To screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. Lac-tobacillus plantarum (HM218749), Lactobacillus reuteri (EU547310). and Enterococcus faecahs (HM218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37℃ in an anaerobic environment and could resist harsh conditions (pH 1.5 and 0.30% bile salt). Lactobacillus plantarum, L. reuteri, and E. faecalis could adhere to HT-29 cells and reduce the adhesion of Shigella dysenteriae 2457, Staphylococcus aureus Cowanl, Enterobacter sakazakii 45401. and Escherichia coli 44102 to HT-29 cells. Moreover, the animal experiment showed that L. plantarum could adhere to mice intestine, increasing the number of lactobacilli and decreasing the number of enterococci.