Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B_1, B_2， G_1 and G_2

摘要

A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B-1, B-2, G(1) and G(2) (AFB(1), AFB(2), AFG(1) and AFG(2)), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed dc-ELISA was 50-230 pg/mL for AFB(1), 50-270 pg/mL for AFB(2), 60-390 pg/mL for AFG, and 65-700 pg/mL for AFG(2). The recovery of AFs from spiked roasted peanuts was 98%. Further, when 4 samples actually contaminated with AFB(1), AFB(2), AFG(1) and AFG(2) were examined, the results of dc-ELISA were highly correlated with the values assigned by the Food Analysis Performance Assessment Scheme. The developed dc-ELISA appears to be suitable for the determination of total AFs at concentrations around the maximum permitted level (10 mu g/kg for all foods) in Japan.