New PCR-RFLP Analysis for the Mouse Tnfsf6~(gld) Gene Caused by a Point Mutation In the Tnfsf6 [Tumor Necrosis Factor (Ligand) Superfamily, Member 6] Locus

摘要

A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6~(gld) mutation. An artificial restriction site was introduced to the mouse Tnfsf6~(gld) mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6~(gld)/Tnfsf6~(gld), Tnfsf6~(gld)/+, and +~(Tnfsf6-gld)/+~(Tnfsf6-gld)) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6~(gld) mutation.