Studies on Cryopreservation of Embryos and Gametes in Mice

摘要

In this study a system for producing young from cryopreserved gametes and embryos has been established (Fig. 5). Especially in gametes, the cryopreserved oocytes could be fertilized in vitro by means of fresh spermatozoa and the cryopreserved spermatozoa could fertilize fresh oocytes and could be used for artificial insemination. Moreover, the cryopreserved oocytes could be fertilized by the cryopreserved spermatozoa and the embryos obtained by in vitro fertilization between cryopreserved gametes could develop into normal, live young after embryo transfer. In the future, if the oocytes and spermatozoa of many mouse strains are frozen separately, the desired embryos and young will be able to be produced from various combinations of cryopreserved gametes.