A quartz crystal microbalance-based Immunosensor for Shrimp Allergen Determination in Food

摘要

A rapid method for determination of food allergen was carried out by quartz crystal microbalance-based immunosensor in this paper, involving the use of polyclonal antibody specific to shrimp allergen based on antibody antigen reaction. Conditions of piezoelectric immunosensor for the analysis of shrimp allergen to be completed in less than 10 min were optimized. The rapid electrode modification was developed on the basis of self-assembly with 1,6-hexanedithiol (HDT) and nanogold. The optimum conditions for detection were as follows: 15 mM HDT was coated on the electrode for 90 min, and nanogold was adsorbed for 110 min at room temperature; the antibody was coated for 3 h under 4 °C, and the coating concentration for anti-shrimp antibody was 0.16 mg/mL, the detecting time was only 500s, and the standard curve between 1 and 300 μg/mL was obtained, in consistent with Sauerbrey equation. The detection limit is 0.333 μg/mL. The recovery of immunosensor was in the range of 96.53–106.22% with coefficient of variation (CV) 6.46–8.89%. Regeneration of the electrode was also studied: piranha and strong alkali and acid had obvious effect, and the electrode can be reused for more than 13 times; glycine had less regeneration capacity for 5 times, but more conveniently. The present immunosensor has showed good performance for application in detection of shrimp allergen.