首页> 外文期刊>Environmental Science & Technology >Shewanella oneidensis MR-1 Chemotaxis in a Diffusion Gradient Chamber
【24h】

Shewanella oneidensis MR-1 Chemotaxis in a Diffusion Gradient Chamber

机译:扩散梯度室中的印度希瓦氏菌MR-1趋化性

获取原文
获取原文并翻译 | 示例
           

摘要

To obtain a systems-level understanding of Shewanella biologyand ecology, the influence of electron acceptor availability on Shewanella s growth, metabolism, and transport needs to be elucidated. The diffusion gradient chamber (DGC) is an experimental tool developed to study population-level microbial growth and motility in response to concentration gradients. In this paper, the response of populations of Shewanella oneidensis MR-1 cells to an applied single gradient of the electron acceptor fumarate and applied opposing gradients of fumarate and nitrate, also an electron acceptor, were studied in the DGC. Mathematical models capable of predicting cellular growth and chemotaxis under the influence of gradients were used to analyze the results. Examining wild-type cells grown in a single gradient of fumarate, we found that MR-1 cells formed a chemotactic band that migrated up the electron acceptor gradient essentially as predicted by the model. The predicted velocity of the chemotactic cell band advancing toward the chemoattractant source (0.139 cm/h, R~2 = 0.996) closely matched that measured in the DGC (0.134 cm/h, R~2 = 0.997). Investigating the impact of opposing gradients of nitrate and fumarate on the chemotactic behaviors of S. oneidensis MR-1 fumarate reductase and nitrate reductase mutants, we found that the DGC was able to separate these mutants based upon their abilities to use the available electron acceptors in accordance with model predictions. Differences in the ability of Shewanella species to respond to and use available electron acceptors is thought to play an important role in their ecology. Therefore, these results validate the use of the DGC system to measure and simulate Shewanella chemotaxis in response to electron acceptor gradients and establish it as a research tool to help elucidate Shewanella s role in environmental processes.
机译:为了获得对希瓦氏菌生物学和生态学的系统级理解,需要阐明电子受体的可用性对希瓦氏菌生长,代谢和运输的影响。扩散梯度室(DGC)是一种实验工具,旨在研究种群水平的微生物生长和响应浓度梯度的运动性。在本文中,在DGC中研究了拟南芥Shewanella oneidensis MR-1细胞群体对电子受体富马酸酯施加的单一梯度以及富马酸盐和硝酸盐(也是电子受体)的相反梯度的响应。使用能够预测梯度影响下细胞生长和趋化性的数学模型来分析结果。检查在富马酸酯的单个梯度中生长的野生型细胞,我们发现MR-1细胞形成了一个趋化带,基本上按照模型的预测向上迁​​移了电子受体梯度。趋向趋化因子源的趋化细胞带的预测速度(0.139 cm / h,R〜2 = 0.996)与DGC中测得的速度(0.134 cm / h,R〜2 = 0.997)非常匹配。调查硝酸盐和富马酸盐的相反梯度对S. oneidensis MR-1富马酸盐还原酶和硝酸盐还原酶突变体的趋化行为的影响,我们发现DGC能够根据其在电子中使用可用电子受体的能力来分离这些突变体与模型预测一致。希瓦氏菌物种对可用电子受体的反应和使用能力的差异在其生态学中起着重要作用。因此,这些结果验证了DGC系统用于测量和模拟响应电子受体梯度的希瓦氏菌趋化性并将其建立为有助于阐明希瓦氏菌在环境过程中作用的研究工具。

著录项

  • 来源
    《Environmental Science & Technology》 |2011年第3期|p.1014-1020|共7页
  • 作者单位

    Department of Chemical Engineering and Materials Science Michigan State University, East Lansing, Michigan 48824, United States,Departments of Microbiology and Molecular Genetics Center for Microbial Ecology Michigan State University, East Lansing, Michigan 48824, United States;

    rnDepartments of Microbiology and Molecular Genetics Center for Microbial Ecology Michigan State University, East Lansing, Michigan 48824, United States;

    rnDepartments of Microbiology and Molecular Genetics Center for Microbial Ecology Michigan State University, East Lansing, Michigan 48824, United States,Departments of Microbiology and Molecular Genetics Michigan State University, East Lansing, Michigan 48824, United States;

    rnDepartment of Chemical Engineering and Materials Science Michigan State University, East Lansing, Michigan 48824, United States,Departments of Microbiology and Molecular Genetics Center for Microbial Ecology Michigan State University, East Lansing, Michigan 48824, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号