Oxygen Consumption Oscillates in Single Clonal Pancretic β-Cells (HIT)

摘要

Based on population studies, we have hypothesized that changes in metabolism in pancreatic β-cells precede changes in Ca~2+. It is well known from single-cell Ca~2+ studies that variable oscillatory patterns in Ca~2+ occur in reponse to glucose stimulation. The present studies, using the clonal β-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concen- tration, insulin secretion, and O_2 consumption and to determine the Ca~2+ dependency of glucose-induced changes in O_2 consumption.