摘要：Banlangen, the dried roots of Radix isatidis, is widely used as an anti-viral agent in China in spite of the questions about its therapeutic mechanism. In our previous work,we noticed that proteins in the roots of Radix isatidis undeiswent glycation through Maillard reaction upon sundrying, and influences on the contents of the extract.These observations made it of interest to isolate and characterize the glycated proteins in the sundried sliced isatis root and to compare them with the corresponding proteins in fresh Radix isatidis.
摘要：Introduction:Both fresh longan and Aiillus longanae have been used in either medicine or food,but they are known to have effects.Like many other TCM, Maillard reaction happened during process from fresh longan to Arillus longanae which may to the difference of their functional effects.Compared with fresh longan,some major disappeared or decreased dramatically in their contents in Arillus longanae.To understand the role of proteins in the glycation process,two proteins with the highest contents in fresh longan were isolated and characterized in this work.
摘要：Background:as a novel antioxidant for the removal of the intracellular PTD-SOD can be useful in reducing intracellular oxidative stress.in this work, the protection and ability of PTD-SOD on L-02 cells damaged by IJVB irradiation was investigated using MTT assay and DNA fragmentations analysis as well as intracellular antioxidant activity detenmination.
摘要：Introduction: The black soybean(Glycine max L.merr)is extensively cultivated in China.Its health benefits are well documented since ancient time,and it is used as a functional diet and even herbal medicine ingredient.Studying its proteins and enzymes is an important aspect to the understanding of its functionality,as well as of its biochemistry of a unique crop.
摘要：Introduction: In our work on reaction products from herbal extracts, we observed that MRPs of radix isatidis could bind to the targeted cell membrane, resulting in cytoprotection effect.To confirm and characterize the binding, a model MRPs of arginine-glucose were prepared and their interaction wig rooster red blood cell was investigated.
摘要：Antiviral agents tend to rely upon specific interactions between the drug and a viral protein, for example sialidase and amantadine, both of which have anti-influenza activity.This specificity secondly,has two disadvantages.Firstly,the drugs have very narrow spectra of activity and,secondly,viruses may develop resistance against them (in the case of amatadine, resistance involved a single amino acid replacement in the M2 protein).
摘要：Background: Enterovirulent E.coli,one of the major foodborne pathogens,contains more than 80 serotypes classified into 6 groups including Enteropathogenic E.coli(EPEC),Enteroinvasive E.coli(EIEC) and Enterohaemorrhaai E. coli(EPEC).The objective of this work was to develop a rapid method for enterovirulent E coli identification the basis of a methodology we proposed for the separation of intact bacterial cells by ion-exchange chromatography.
摘要：IntroduMion:Maillard reaction is nonenzymatic reaction between reducing sugars and free amido group in amino acids or proteins,resulting in a complex series of compounds called Maillard reaction products (MItPs).M1tPs have been shown to possess potent antioxidant activity, which is not only important to food storage,food processing and herbal medicine preparation but also to the understanding of plant antioxidant system.In this study, model MRPs were prepared from arginine-glucose under different conditions to elucidate the factors influencing MRPs'antioxidant properties.
摘要：Radix Isatidis,the root of the plant Isatis indigotica Fort.or Isatis tinctoria L.,and bitter melon (Momrodica Charantia L.)are widely used in China as antiviral and antidiabetic agents,respectively.Arginine and Glucose is the dominant ent in their free amino acids and reducing sugar The Maillard reaction products(MRPs)of these two plants and Arginine-Glucose were prepared by heating process to obtain RIE,MCE and HIvIRPs.The interaction of these MRPs and the stack of membrane phospholipids DOPC bilayers was investigated by neutron scattering.
摘要：Introduction:The bitter melon(Momordica charantia L)is extensively cultivated in China and some Asian countries.Its is not only a common daily vegetable but is used as a functional vegetable as a folk remedy for conditions such as diabetes, cancer and obesity in many countries.Numberous enzymes and bioactive proteins have been identified in bitter melon fruit and seed,further work is required in order to validate and elucidate its health benefits.
摘要：The antidiabetic effects of the decoction of bitter melon(Momordica Charantia Linn.)pulps has been proven in vitro and in vitro by our previously studies,mainly on Type B diabetic subjects.The major active component of the decoction is Maillard reaction products (MRPs).The damage on pancreatic cells caused by free radicals is one of the major cellular pathological changes in diabetes.The effects of MRPs on this free radical induced cellular change was studied in order to illuminate the mechanism of MRPs'antidiabetic effects.
摘要：本文为探索有关微生物积累油脂的意义，对Lipomyces starkeyi AS 2.1560的摇瓶产脂发酵特性进行了研究.通过极差分析，可以得出各因子影响大小次序为:接种量＞C/N＞发酵周期＞装液量＞pH;接种量为10%、初始pH为6、发酵周期为6d、C/N为70:1、装液量为65mL/250mL三角瓶为最适发醉条件.最终生物量达18.84g/L，油脂量达3.58g/L.
摘要：Chemotherapy-induced myelosuppression and aplastic anemia are bone marrow failure syndromes characterized by peripheral pancytopenia and marrow hypoplasia.Few good therapeutics have been developed to solve the problems.We have identified a novel peptide(PP),derived from serum albumin,with a potent activity to stimulate hematopoietic cells proliferation.Assay of proliferation in vitro was carried out with human haematopoietic stem cells (CD34+)and bone marrow nucleated cells from healthy volunteers and aplastic anemia patients'bone marrow (donors), respectively.
摘要：Introduction: Radix Isatidis is the most important antiviral herbal ingredient in China as an ingredient in a compound medicine or herbal tea,which is prepared from the boiling water extract of the sundried root of Isatis indigotica Fort.Considering the remarkably high contents of proteins and reducing sugars in most herbs,it is reasonable to assume that Maillard reaction occurs when herbs are heated during sundrying.In this work,the influence of sundrying on the protein content and trypsin inhibitory activity of the extracts were investigated in order.to evaluate the impact of Maillard reaction.
摘要：Background:The advent of chromatographic methods has significantly revolutionized studies of the chemistry of many fields by their efficient and convenient separation of molecules,thereof the feasibility of separating intact bacterial cells by ion exchange chromatography typical for macromolecular study was investigated in this paper.A common HPLC system coupled with a light scattering detector was employed;TOYOPEARL SuperQ-650C, an anion exchanger, was used as the column media.
摘要：Inroduction:Antifungal proteins play an important role in the defense of crops against fungal infection.Identification of those antifungal proteins is vital to the understanding of the natural defense mechanism of plants and crops.It can also provide useful clues in the development of biologically effective and environment-friendly pest management agents to replace traditional chemical pesticides.Our objective in this study was to isolate novel proteins with antifungal activity from black soybean.
摘要：Arillus longanae, dried longan, has been used as function food for hundreds of years in China.It is listed as a herb which can be used as both food and medicine by Chinese Ministry of health.Arillus longanae has bioactive properties differed from fresh longan,such as blood enriching, antioxidant,immunity enhancing and endocrine influencing activities.It appears that the heating process provides not only a preservative means but also the generation of new bioactivities in longan.The aim of this study is to investigate the antioxidant activities of the Arillus longanae due to the heating processing.
摘要：UV B irradiation induced cutaneous damage is the result of cellular oxidative stress.As a dismutase which can traverse across the cell membrane to quench potent inside the cell,PTD-SOD was expected to be effective prevention or alleviation of cutaneous damage caused by LTVB.This study by to UVB.This study was to verify the assumption.
摘要：Introduction:Antifungal proteins and peptides in plants,as their names imply,play an important role in the defense of crops against fungal attack which can bring about serious economic losses.Legumes are a good resource for the screening of those proteins and peptides for the high content of storage proteins particularly require such a defense system.Our objective in this study was to isolate novel proteins with antifungal activity from black soybean.
摘要：PTD-SOD is a reombinant antioxidant enzyme we prepared by fusing a protein transduction domain with hCu,Zn-SOD, with capability to traverse into cell to remove.haddition to its prominent intracellular antioxidant activity, we also notice PTD-SOD's whitening effect on skin.To understand its mechanism, the PTD-SOD's tyrosinase activity was investigated in vitro using dopa, and the skin whitening effect was studied using mice model.
摘要：Introduction:Bitter melon extract has been used in many Asian countries as a herbal remedy for diabetes, tumor, A)DS,and immunity boosting.One of the most prominent properties of bitter melon extract is its potent antioxidant activity which may be possibly responsible for its therapeutic effect.In this study,influence of extraction conditions on the antioxidant activity of the extract was investigated.
摘要：本文以厦门树白蚁为原料，经PBS缓冲液抽提、硫酸按分级分离获得粗酶制剂，进一步经DEAF-Sepharose Fast Flow阴离子交换柱层、Shephadex G-100凝胶过滤柱层析等纯化，得到电泳单一纯的纤维素酶制剂，比活力为458.7U/mg。研究表明，苯酚对该酶的半抑制浓度为0.25mol/L,当其浓度达0.89 mol/L.酶的剩余活力仅为9.5%。而米氏常数(Km)则随着苯酚浓度增大而增大，其抑制机理表现为竞争性类型，苯酚和底物(S)竞争游离酶的结合部位，但不能和结合酶结合。其抑制常数(KIS)为0.64mol/L。
