Biocompatibility of the titanium-based implant surfaces: Effect of the calcium dihydrogen phosphate on osteoblast cells

摘要

The influence of the presence of calcium dihydrogen phosphate in acid media on titanium-based implant surfaces, Ticer, employed in clinics, and its white form (Ticer white), on osteoblast cells was investigated. Novel surfaces M1 and M2 were obtained by immersing Ticer and Ticer white surfaces in calcium dihydrogen phosphate solution at pH 3.5. The surfaces were characterized by SEM, EDS and X-ray diffraction. The results related to interaction of investigated surfaces and human osteoblast cells from indirect biocompatibility (MTT and SRB assays), proliferation (DAPI assay) and mode of cell death (acridine orange/ethidium bromide (AO/EB) double staining) were found to be in good agreement, as well as findings from osteocalcin (OC) and bone sialoprotein (BSP) expression. Surfaces were obtained by employing anodic plasma-electrochemical oxidation with spark discharges without subsequent surface modifications were found to be more compatible. Soaking of Ticer and Ticer white in phosphate solution gave toxic materials (M1 and M2) which induced apoptosis and secondary necrosis in osteoblast cells.