目的: 探讨不同粗糙度氧化锆表面经紫外线处理,对小鼠胚胎成骨细胞前体细胞(MC3T3-E1) 生物学行为的影响.方法: 将氧化锆试件先行抛光和喷砂,处理组用15 W[波长(250 ± 20) nm]灭菌灯对试件照射48 h,分为光滑对照组(S组) 、光滑处理组(S-UV组) 、粗糙对照组(R组) 和粗糙处理组(R-UV组) 4组(n = 3),将MC3T3-E1细胞分别培养于试件表面.MTT法检测接种3、5 d细胞增殖率,3、7 d时检测细胞分泌碱性磷酸酶(ALP) 的活性;茜素红染色培养21 d细胞,检测钙结节形成情况;RT-PCR法检测培养7、14 d骨相关蛋白骨钙素(OCN) 、护骨素(OPG) mRNA的表达.结果: 紫外线处理组比对照组更利于MC3T3-E1细胞的增殖与矿化,R-UV组与其他组差异明显(P ＜ 0. 05),7 d组的ALP活性高于3 d组(P ＜ 0. 05),7 d时R-UV组ALP活性均高于S组和S-UV组(P ＜ 0. 05),粗糙组间无明显差异.RT-PCR显示,R-UV组OCN及OPG mRNA的表达明显上调(P ＜ 0. 05).结论: 氧化锆表面经紫外线处理后可促进成骨细胞在其表面增殖、矿化,上调OCN、OPG mRNA的表达.%Objective: To study the effects of UV treatment of different zirconia surfaces on the function of MC3T3-E1 osteoblasts. Methods: Disks of zirconia-based materials with smooth(S) and rough(R) surfaces were treated and with and without UV light(15 W) for 48 h,respectively. MC3T3-E1 cells were cultured on zirconia disks and divided into 4 groups: smooth group(S),UV treated smooth group(S-UV),rough group(R) and UV treated rough group(R-UV). Cell proliferation was assessed by MTT 3 and 5 days after culture. Alkaline phosphatase(ALP) activity was determined 3 and 7 days after culture. Cell mineralization was observed by Alizarin red dye staining 21 days after culture. The mRNA expression of OCN and OPG were examined by RT-PCR 7 and 14 days after culture. Results: Proliferation and mineralization of MC3T3-E1 cells in R-UV group were more than in other groups(P ＜ 0. 05). R groups showed higher ALP activity than S groups at day 7. R-UV treatment increased the mRNA expressions of OPG and OCN in a time-dependent manner(P ＜ 0. 05). Conclusion: Ultraviolet light-treated zirconia surface may promote the proliferation,mineralization and the expression of OCN and OPG of MC3T3-E1 cells.
展开▼
