Effect of Different Carbon and Nitrogen Sources on the Growth of Two Thermophilic Actinomycetes and Their Cellulases Production

摘要

Thermophilic cellulases have emerged as promising alternatives in biotechnological applications. At present, most of the enzymes used in industrial processes have been isolated from mesophiles microorganisms due to the difficulties to grow thermopiles in high scale. The present investigation deals with optimization of the production medium with different carbon and nitrogen sources to observe their effect on the growth and production of extracellular proteins, reducing sugar, saccharification percentage, biomass yield and cellulases of the selected isolates at high temperature. In the present study, the isolate Streptomyces longisporus (A_(1)) showed maximum production (activities) of CMCase (265.75 U/ml) and Avicelase (86.61 U/ml) in the presence of lactose and fructose as suitable carbon sources respectively; on the other hand the isolate Streptomyces bobili (A_(4)) exhibited maximum production (activities) of FPase (177.16 U/ml) and β-glucosidase (209.65 U/ml) with galactose. KNO_(3 )was found suitable nitrogen source for CMCase production (activities) of the both isolates. These findings will be helpful in prospect for increase the production of industrial enzyme.