The performance of the SD BIOLINE Dengue DUO? rapid immunochromatographic test kit for the detection of NS1 antigen, IgM and IgG antibodies during a dengue type 1 epidemic in Jamaica

摘要

BackgroundDengue is an important mosquito-borne viral infection that affects millions of persons worldwide. Early diagnosis is necessary to effect appropriate management and decrease mortality. Immunochromatographic tests are advantageous in producing dengue test results within 30?min but these results should be sensitive and specific. In this study we evaluated the diagnostic performance of the SD BIOLINE Dengue DUO? rapid immunochromatographic test kit. A panel of 309 dengue and 30 non-dengue single serum samples characterized by using reference enzyme-linked immunosorbent assays (ELISAs) was used. These samples were received in the virology laboratory for routine testing during a dengue type 1 outbreak between October to December, 2012.ResultsThe overall diagnostic sensitivities of the SD BIOLINE Dengue DUO? rapid testfor IgM, IgG and NSI were 49.3?% (95?% CI: 41.3-57.4), 39.1?% (95?% CI: 33.3-45.2) and 90?% (95?% CI: 82.1-94.7), respectively. The IgM and IgG detection rates were significantly lower than that of the NSI (p?