Elevated Plasma Adiponectin Level and Peripheral Blood LeukocyteAdiponectin Receptor Expression in Dogs Suffering from InsulinDeficiency

摘要

The aim of this study is to determine and compare the effect of obesity or insulin deficiency on plasmaadiponectin level and peripheral blood leukocyte (PBL) adiponectin receptor 1 and 2 (ADIPOR1 and -R2) expression indogs. Obese and insulin deficient (ID) dogs demonstrated a significant reduction (60% lower) and significant increase (2xhigher) in circulating adiponectin level, respectively, as compared to normal controls. PBL ADIPOR1 and -R2 mRNAexpression was also significantly higher in obese (R1=333 mean fold higher, R2=64 mean fold higher) and ID dogs preinsulintreatment (R1 =48 mean fold higher, R2=16 mean fold higher) as compared to normal PBL. Insulin treatmentreduced ADIPOR1 (3 mean fold higher) and -R2 (1.5 mean fold higher) expression back down closer to control PBLlevels in ID animals. The upregulation of adiponectin receptor expression might reflect an increased need for adiponectinsignaling; however, the increase may have a different implication between obese and ID dogs. This may be due toadiponectin's contradictory paradoxical dual role, having both anti-inflammatory and pro-inflammatory properties onperipheral leukocytes, especially on monocytes depending on the biological context and adiponectin isoform.