Survivability of boar sperm stored under room temperature in extenders containing some natural products

摘要

Background: Semen extenders are used to create multiple insemination doses from a single ejaculate and contain buffers and nutrients suitable for storage of spermatozoa. The purpose of this study was to evaluate the survivability of boar sperm stored under room temperature in different extenders containing some natural products. Methods: A volume of 10 mL of pooled semen collected from four mature crossbred (Landrace × Large White) boars was stored in 12 different extenders containing 1.0%, 1.5%, and 2.0% per liter each of honey, sugarcane juice, tomato juice, and pineapple juice. The semen samples were observed under a microscope every hour to determine sperm motility by scoring. Survivability of the sperm cells was taken as the average percentage number of motile spermatozoa in each extender recorded during 24 hour storage duration. The experimental layout was a 4×3 factorial in a randomized complete block design. Results: Average sperm survivability was higher for semen stored in honey and sugarcane juice extenders compared (P<0.05) to sperm stored in tomato and pineapple extenders. The proportion of spermatozoa that survived within the 24 hour storage time was highest in the honey group and lowest in the pineapple group. The average percentage of sperm that were motile was higher when 1.5% and 2.0% of test ingredients was included in extender compared (P<0.01) to 1.0% inclusion rate. Conclusion: The present study clearly demonstrates that spermatozoa can be stored in extenders containing honey, sugarcane juice, tomato juice, and pineapple juice. Moreover, the survivability of sperm in these extenders can be enhanced when the percentage constituent of these natural materials in the extenders is increased from 1.0% to 2.0% inclusion rates.