Antioxidant and anti-tumor activities of pectinesterase inhibitor fractions from jelly fig (Ficus awkeotsang Makino) achenes

摘要

The aims of this study are to observed antioxidant activities of jelly fig fractions (ferrous ion chelating activity, DPPH radical scavenging activity, and reducing power) and anti-proliferative activity against K562 myeloid leukemia cells. The jelly fig fractions were obtained by separating polyphenol and protein from pectin esterase inhibitor (PEI) extract into crude polyphenol (CP) and protein fraction (PR). CP was further purified by liquid chromatography into purified polyphenol (PP). The results showed that PP fraction had the highest antioxidant activities and anti-proliferative activity against K562 myeloid leukemia cells, while PR fraction had the lowest. All fraction of jelly fig had higher ability (lower EC50 values) in DPPH radical scavenging activity than ferrous ion chelating activity. Comparing the IC50 of anti-proliferative activity of different fractions of jelly fig after 24 h treatment, PP fraction had the lowest value (27.27 -g/mL), followed by CP (85.89 -g/mL), PEI (144.47 -g/mL) and PR (too low to be determined). Polyphenol of jelly fig showed promising bioactivities against oxidation and leukemia cell proliferation.