High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels

O’Connell Grant C; Chantler Paul D; Barr Taura L

首页> 外文期刊>Laboratory medicine >High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels

【24h】

High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels

机译：核酸提取效率中的高种间变异性需要使用尖峰控制进行基于qPCR的血浆无细胞DNA水平的准确测量

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

To assess the interspecimen variability associated with plasma DNA extraction in order to provide insight regarding the necessity to use an exogenous spike-in control when measuring cell-free DNA (cfDNA) levels using quantitative polymerase chain reaction (qPCR).

机译：为了评估与血浆DNA提取相关的种间变异性，以便提供有关在使用定量聚合酶链反应（qPCR）测量无细胞DNA（cfDNA）水平时使用外源刺入对照的必要性的见解。

著录项

来源
《Laboratory medicine》 |2017年第4期|共7页
作者
O’Connell Grant C; Chantler Paul D; Barr Taura L;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类病理学;
关键词

相似文献

外文文献
中文文献
专利

1. Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification [J] . Alison S. Devonshire, Alexandra S. Whale, Alice Gutteridge, Analytical and bioanalytical chemistry . 2014,第26期

机译：实现血浆中无细胞DNA测量的标准化：提取效率，片段大小偏差和定量的控制
2. Liquid Biopsies Using Plasma Exosomal Nucleic Acids and Plasma Cell-Free DNA Compared with Clinical Outcomes of Patients with Advanced Cancers [J] . Lino M?hrmann, Helen J. Huang, David S. Hong, Clinical cancer research: an official journal of the American Association for Cancer Research . 2018,第1期

机译：液体活组织检查使用血浆外泌核酸和血浆无细胞DNA与晚期癌症患者的临床结果进行比较
3. Nucleic Acids Research Group (NRG): The Importance of DNA Extraction in Metagenomics: The Gatekeeper to Accurate Results! [J] . R. Carmical, V. Nadella, Z. Herbert, Journal of biomolecular techniques :JBT. . 2013,第Suppl期

机译：核酸研究组（NRG）：DNA提取在超基因组学中的重要性：精确结果的看门人！
4. Novel DNA Extraction and Preservation for Identification of Micro-organisms On-site using Novel Nucleic Acid Extraction Cards for the Oil and Gas Industry [C] . Andy Price Corrosion conference and expo;Corrosion 2012 . 2012

机译：用于石油和天然气行业的新型核酸提取卡，用于现场鉴定微生物的新型DNA提取和保存
5. Plasma DNA Sequencing: A Tool for Noninvasive Prenatal Diagnosis and Research into Circulating Nucleic Acids. [D] . Zheng, Wenli. 2010

机译：血浆DNA测序：无创产前诊断和循环核酸研究的工具。
6. High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels [O] . Grant C O’Connell, Paul D Chantler, Taura L Barr -1

机译：核酸提取效率中的高种间变异性需要使用尖峰对照进行基于qPCR的血浆无细胞DNA水平的准确测量
7. Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification [O] . Alison S. Devonshire, Alexandra S. Whale, Alice Gutteridge, 2014

机译：努力实现血浆中无细胞DNA测量的标准化：提取效率，片段大小偏差和定量的控制

High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels

摘要

著录项

相似文献

相关主题

期刊订阅