Effect of phytocercaricide on certain enzyme activity in parasitic cercaria larva of Fasciola gigantica

摘要

Fasciolosis is one of the common public health problems worldwide, and cause a great economic loss to us. The life cycle of the Fasciola can be interrupted by killing the vector snail or Fasciola larva redia/cercaria inside the snail body. Binary combinations of different plant derived cercaricidal were more effective against the cercaria larva of Fasciola gigantica. Treatment with binary combination 20% and 60% of 4h LC50 of citral (Ci), ferulic acid (Fe), umbelliferone (Um), azadirachtin (Az) and allicin (Al) caused no significant change in the activity of acetylcholinesterase (AChE) in the nervous tissues of exposed L. acuminata. Significant inhibition in the AChE activity was noted in whole cercaria larva. AChE activity in the nervous tissues of snail exposed to 60% of 4h LC50 of Al+Um was 94.50% of control. AChE and cytochrome oxidase activity was measured to reduced 0.89% and 2.37% of control in whole cercaria larva exposed to 60% of 4h LC50?of Az+Al.