Effects of Angelica polysaccharide on erythrocyte immunity and marrow hematopoiesis in chickens

摘要

To investigate the effects of?Angelica?polysaccharide on erythrocyte immunity and marrow hematopoiesis in chickens,?Angelica?polysaccharide was used for the study of immunity and hematinic mechanism in order to provide basis for clinical reference. In this experiment, three gradient dosages (50, 100, 150 mg/kg body weight) of?Angelica?polysaccharide were drenched to the control group and the anemia groups, respectively. The anemia chickling model was made by abdominal injection of cyclophosphamide (CY) for 6 days (80 mg/kg·day).Red blood cell-C3b receptor (RBC-CR1) and red blood cell-immune complex (RBC-IC) rosette rates were measured and analyzed. Ectogenetic semi-solid culture medium of bone marrow hemopoietic progenitor cells was used to observe?Angelica?polysaccharide and separated serum fromAngelica?polysaccharide?treatment?on the proliferation of colony-forming unit-erythrocyte (CFU-E), burst-forming unit-erythrocyte (BFU-E) and colony-forming unit-granulocyte macrophage (CFU-GM). The results showed that?Angelica?polysaccharide can significantly increase RBC-CR1 rosette rate in the healthy chicken groups (p0.05). At the same time,?Angelica?polysaccharide can restore the RBC- CR1 rosette rate and the RBC-IC rosette rate caused by cyclophosphamide to the normal level. Serum containing?Angelica?polysaccharide can significantly facilitate the proliferation on CFU-E (p<0.01), BFU-E (p<0.01) and CFU-GM (p<0.01), but?Angelica?polysaccharide had no more direct proliferation on CFU-E, BFU-E and CFU-GM. This indicated that?Angelica?polysaccharide had the proliferation on hemopoietic progenitor cells of marrow by the change of hemopoietic factor.