EFFECT OF VARIOUS CONCENTRATIONS OF MINIMAL ESSENTIAL MEDIUM VITAMINS (MEM VITAMINS) ON DEVELOPMENT OF SHEEP OOCYTES DURING IN-VITRO MATURATION

摘要

Background: Improvements in culture media formulations have led to an increase in the ability of sheep embryo in culture throughout the preimplantation period.Objective: This study was carried out to evaluate the effects of various concentrations of MEM vitamins during in vitro maturation of sheep oocytes and subsequent embryo development.Materials and Methods: Sheep ovaries were collected from a slaughterhouse and transported to the laboratory. Oocytes were matured in SOF medium supplemented with, eCG, hCG and EGF in various concentrations of MEM vitamins (control, 0.5, 1 and 1.5