Evaluating the Sensitivity of Three Primers Using PCR-Restriction Fragment Length Polymorphism Analysis for Rapid Identification of Mycobacterium Simiae Isolated from Pulmonary Tuberculosis Patients

摘要

Background: Nowadays the molecular methods widely use for rapid identification of Mycobacterium other than tuberculosis (MOTT). The Mycobacterium simiae isolates are cause of majority of human pulmonary diseases compared with other atypical mycobacteria. As sensitivity of primers and digestion patterns for diversified fragments is different, this survey evaluated the three various fragments using the PCR- restriction fragment length polymorphism analysis (PRA) for rapid diagnostic of M. simiae isolates.