Square-Wave Adsorptive Stripping Voltammetric Determination of Serotonin at Glassy Carbon Electrode Modified with Safranine O

摘要

A poly(safranine O) modified glassy carbon electrode has been prepared by an electrooxidativepolymerization of safranine O. The electrochemical behavior of serotonin (5-HT) at this modifiedelectrode was investigated by cyclic voltammetry (CV) and square wave adsorptive strippingvoltammetry. The modified electrode was employed as an electrochemical sensor for the detection ofserotonin concentration and exhibited a typical enhance effect on the current response of serotonin andlower oxidation overpotential. Due to the relatively low currents and different potentials in theelectrochemical responses to ascorbic acid and dopamine, the modified electrode is a useful andeffective sensing device for the sensitive and selective serotonin determination in the presence ofascorbic acid and dopamine. At optimised conditions, the calibration curve for serotonin was showed -1 two linear segments: the first linear segment increased from 3.0 10 to 1.0 10 mol L and -1 second linear segment increased up to 1.0 10 mol L . The detection limit was determined as 5.0-9 -1 10 mol L using SW-AdSV. The proposed method was successfully applied to determine serotoninin human serum samples.