In vitro evaluation of cytotoxic effect of elastomeric chain disinfected with glutaraldehyde 2% and peracetic acid 0.25% on L929, fibroblast and Helacell lines

摘要

Background and objective:In Orthodontics, elastomeric chains are in contact with different types of material thatcan increase the risk of contamination.Sterilization using peracetic acid (PAA) and glutaraldehyde has beencommonlyutilized for orthodontic elastic bands. In this study, the cytotoxic effect of elastomeric chainsdisinfected with glutaraldehyde2% and peracetic acid 0.25% on different cell lines was investigated.Material and methods:Orthodontic elastomeric chainswith 28 links weresterilized in 0.25% PAA and 2%glutaraldehyde at time intervals of 1 hour, 6hours, and48 hours. In the following, disinfected elastomeric chainswere washed using distilled water for 15 second, 24 hours and without washing. After this, the elastics wereimmersed in RPMI medium for 24 h and 72 h torelease possible toxic substances. Then, the medium was placedincontact with L929, fibroblastand HELA cells for 24 h. Finally, cell viability was evaluated by MTT assay.Results:Our results demonstrated that the highest decrease in cell viability was seen in all cell lines in disinfectionwith glutaraldehyde without washing. In addition, the viability of the treated cell lines usingglutaraldehydeincreased with increasing the washing time and the highest viability was observed in 24h washingtime. The viability of all cell lines in disinfection by peracetic acid was significantly higher in comparison withdisinfection using glutaraldehyde in all conditions especially without washing. Moreover, the change in themorphological characteristics of all cell lines in disinfection with peracetic acid was significantly lesser incomparison with disinfected using glutaraldehyde in all states especially without washing.Conclusion:In the present study, peracetic acid proved an efficient disinfectant agent, in addition to not affectingthe viability of the treated cell lines, therefore recommended for clinical use.