Characterization of mid-intestinal microbiota of farmed Chinook salmon using 16S rRNA gene metabarcoding

摘要

Paper description: With worldwide growth of aquaculture, the characterization of microbiota of high-value aquaculture species is of special interest.This paper reports a novel workflow for high-throughput surveys of bacterial intestinal microbiota of aquacultured fish using metabarcode profiling of the V3-V4 hypervariable region of the bacterial 16S rRNA gene.The workflow was used to produce the first 16S rRNA gene metabarcoding survey of the mid-intestinal microbiota of farmed Chinook salmon.The presented workflow could be applied to other aquacultured fish species to capture variation or dysbiosis occurring as a result of changes in feed, health or environmental conditions.