Riboflavin may ameliorate neurological motor disability but not spatial learning and memory impairments in murine model of multiple sclerosis

摘要

Background & aimsRiboflavin has an important role in myelin formation. This experimental study assesses the interactions between the effects of riboflavin and interferon beta-1a (INF-β1a) on motor disability, spatial learning and memory, and brain-derived neurotrophic factor (BDNF) in experimental autoimmune encephalomyelitis (EAE).MethodsIn the present research C57BL/6 mice (n?=?56) were divided into sham and treatment groups. Riboflavin was administrated (10?mg/kg/day) orally for two weeks alone and/or combined with INF-β1a at 150 IU/g of body weight. After the induction of EAE, the animals were investigated for the clinical signs. Spatial learning and memory were assessed through the standard Morris water maze (MWM). The brain and spinal cord levels of BDNF were studied using real-time polymerase chain reactions and enzyme-linked immunosorbent assay. The data were analyzed using one-way ANOVA, repeated measures, and generalized estimating equations model.ResultsThe results in the brain revealed that BDNF mRNA expression (P?