Primary Culture of Choroid Plexuses from Neonate Rats Containing Progenitor Cells Capable of Differentiation

摘要

Background: The choroid plexuses, which could secrete a number of neurotrophins, have recently been used in transplantation in central nervous system diseases. Aims: To study the mechanism of nerve regeneration in the central nervous system by grafting choroid plexus tissues. Study Design: Animal experimentation. Methods: The choroid plexuses from the lateral ventricles of neonatal rats were cultured in adherent culture, and immunocytochemical methods were used to analyse the progenitor cells on days 2, 6, and 10 after seeding. Results: Expression of both nestin and glial ?brillary acidic protein was observed in small cell aggregates on day 2 in primary culture. Most of the nestin-positive cells on day 6 were immunoreactive to glial ?brillary acidic protein antibody. No cells expressing nestin or glial ?brillary acidic protein were seen on day 10. Conclusion: These experimental results indicate that the choroid plexus contains a specific cell population – progenitor cells. Under in vitro experimental conditions, the progenitor cells differentiated into choroid plexus epithelial cells but did not form neurons or astrocytes. Turkish Ba?l?k:Farkl?la?ma Kabiliyeti Olan Progenit?r Hücreler ??eren Yenido?an Ratlar?n Koroid Pleksusunun Primer Kültürü Anahtar Kelimeler:Koroid pleksus, primer kültür, progenit?r hücreler, farkl?la?ma, rat Arkaplan: Bir dizi n?rotrofin salg?layabilen koroid pleksus, son zamanlarda merkezi sinir sistemi hastal?klar?nda transplantasyonda kullan?lmaktad?r. Ama?: Koroid pleksus dokular?n?n greftlenmesi ile merkezi sinir sisteminde sinir rejenerasyonunun mekanizmas?n? incelemek. ?al??ma Tasar?m?: Hayvan deneyi Y?ntemler: Yenido?an ratlar?n lateral ventrikülünden al?nan koroid pleksuslar yap???k (adherent) kültür ile ?o?alt?ld? ve immünositokimyasal metotlar ekim sonras? 2., 6. ve 10. günlerde progenit?r hücrelerin analizi i?in kullan?ld?. Bulgular: Primer kültürde 2. günde kü?ük hücre kümelerinde hem nestin hem de glial fibriler asidik protein ekspresyonu g?zlendi. 6. Günde nestin-pozitif hücrelerin ?o?u, glial fibriler asidik protein antikoruna kar?? immunoreaktif idi. 10. Günde nestin veya glial fibriler asidik protein eksprese eden hücre g?rülmedi. Sonu?: Bu deneysel sonu?lar, koroid pleksusun spesifik bir hücre popülasyonu-progenit?r hücreleri i?erdi?ini g?stermektedir. In vitro deneysel ko?ullar alt?nda, progenit?r hücreler koroid pleksus epitel hücrelerine farkl?la?m?? ancak n?ronlar? veya astrositleri olu?turmam??t?r.