The Role of Hsp90αin Apoptosis and Damages of Primary Myocardial Cell Cultures of Neonatal Rats after Heat Stress

摘要

This study describes the relation among the heat induced pathological changes and apoptosis,and the variations of protective Hsp90α and its corresponding mRNA in the heat stressed primary myocardial cells of neonatal rats in vitro.Levels of enzyme CK increased from the beginning of heat stress and acute cellular lesions characterized by vacuolar degeneration and necrosis in the heat stressed cells were observed after 40 min of heat stress,suggesting that the myocardial cells in vitro were obviously damaged by higher temperature.The levels of cleaved Caspase-3 and Cytochrome C which concerned to apoptosis increased significantly after 40 min of heat stress while Hsp90α protein significantly decreased,and the levels of cleaved Caspase-3 and Cytochrome C decreased while Hsp90α significantly increased after 6 h of heat stress,indicating that early depletion of Hsp90α coincides with a high rate of necrosis and apoptosis in heat stressed myocardial cells while in surviving cells Hsp90α increases after 6 h of heat stress again with significantly less apoptosis.