A Combined Peritoneal Macrophage/Cell Membrane Chromatography and Offline GC–MS Method for Screening Anti-Inflammatory Components from Chinese Traditional Medicine Houttuynia cordata Thunb.

摘要

The aim of this study was to screen for the anti-inflammatory components from Houttuynia cordata Thunb (HCT) using an analytical method combining cell membrane chromatography (CMC) with gas chromatography–mass spectrometry (GC–MS). The stationary phase of the CMC employed mouse peritoneal macrophage (PM) cell membranes. We investigated the performance of the PM/CMC-offline-GC–MS method using hydrocortisone (HC) as standard. The method was then applied to the identification of anti-inflammatory components in extracts of HCT. The major component retained by CMC was identified as methyl nonyl ketone (MNK) by GC–MS. In vitro experiments revealed that MNK was able to inhibit LPS-induction of TNF-α, NO, and H₂O₂ production in a dose-dependent manner. IC₅₀ values were 3.3, 4.1, and 3.6 μg mL−1, respectively. The PM/CMC-offline-GC–MS method is an effective screening system for the rapid detection, enrichment, and identification of target components from complex samples.