The effect of DNA aptamer configuration on the sensitivity of detection thrombin at surface by acoustic method

摘要

Using an acoustic method we showed that the aptamer configuration, especially those comprised of dimers with binding exosites sensitive to heparin and fibrinogen at thrombin, substantially increases the aptasensor sensitivity.rnDNA aptamers are single stranded oligonucleotides (< 100 bases) with high affinity to proteins or other ligands, comparable to those of antibodies. The aptamers are selected in vitro by the SELEX method. Once the sequence is selected, aptamers can be multiplied with high precision and purity. In solution, the selected sequence maintains an unique 3D configuration that contains a specific binding site for the ligand. Aptamers can be easily modified by biotin, SH or amino groups, leading to a variety of immobilization strategies on solid supports. Thus, aptamers can serve as simple and highly sensitive artificial receptors.