核酸适体在自组装法制备石墨烯/金纳米复合薄膜上的固定及凝血酶的检测

摘要

In the present work,the nanocomposite of graphene/Au nanoparticles (g/AuNPs)was prepared by the method of self-assembling approach and used as the bioactive layer for aptamer immobilization and thrombin detection.The surface morphology of the nanocomposite layer was characterized by atom force microscopy.And the chemical component of the multilayers of g/AuNPs was investigated by X-ray photon spectroscopy.The variations of the electrochemical properties of g/AuNPs were determined by the electrochemical working-sta-tion.Moreover,the mass change in the period of the self-assembling was measured by quartz crystal microbal-ance in situ.It demonstrates that the nanocomposite of g/AuNPs could be used as the bioactive layer for aptam-er immobilization and thrombin detection.%采用自组装法制备出石墨烯/金纳米(g/AuNPs)复合材料，并将核酸适体固定其表面上，用于凝血酶的检测。采用原子力显微镜对 g/AuNPs 自组装膜的表面形貌进行了详细考察，采用 X射线光电子能谱对核酸适体在 g/AuNPs 自组装膜上固定前后的元素组成进行测试。同时对核酸适体固定前后 g/AuNPs自组装膜的电化学性能变化进行了测试。采用石英晶体微天平对g/AuNPs自组装、核酸适体固定和凝血酶检测过程中的 g/AuNPs 复合材料薄膜质量变化进行在位检测。结果表明，采用简单易行的自组装法所制备出的 g/AuNPs 复合材料有望使用在核酸适体生物传感器方面。