establishment of an artificialβ-cell line expressing insulin under the control of doxycycline

摘要

Conclusion: Human proinsulin gene was transfected successfully and expressed efficiently in 293 cells, and the expression was modulated by tetracycline and its derivatives, improving the accuracy, safety, and reliability of gene therapy, suggesting that conditional establishment, of artificialβ-cells may be a useful approach to develop cellular therapy for diabetes mellitus. Aim: Artificialβ-cell lines ma offer an abundant source of Cells for the treatment of type I diabetes, but insulin Secretion inβ-cells is tightly regulated in physiological Conditions.