In vitro antitumor activity of titanocene alanine complex and its interaction with nucleic acids

摘要

Titanocene alanine complex, Cp_2Ti(O_2CCH(CH_3)NH_3~+Cl~-)_2, has been found to have in vitro antitumor activity against Ehrlich ascites tumor cells. Study of the interaction between Cp_2Ti(O_2CCH(CH_3)NH_3~+Cl~-)_2 and DNA by UV-VIS spectra, fluorescence spectra, agarose gel elec-trophoresis reveals that Cp_2Ti(O_2CCH(CH_3)NH_3~+Cl~-)_2 forms a stable compound with DNA in molar ratio 1:1 and reduces the migration rate of ccc-DNA band and oc-DNA band. However, unlike cis-platin, the complex does not induce nicking of DNA even after a long incubation at a high ratio of the complex to DNA. The results suggest that titanocene-based antitumor agents have different mechanism of action from cisplatin-like antitumor agents.