Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina E. tenella or E. necatrix

摘要

sporozoite protein 2-dimensional gel electrophoresis (2-DE) profile demonstrated by silver staining. The soluble sporozoite proteins (200 μg) of were first resolved by isoelectric focusing (IEF) using a 24 cm immobilized pH gradient (IPG) strip (pH 3–10). Then, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (12.5%) was used to separate the proteins by size followed by silver staining. The Y-axis shows the protein Mr. range between 11 and 180 kDa and X-axis indicates the protein pI range between 3 and 10. Data represent results from 2 independent experiments