The Role of Urine in Semiochemical Communication between Females and Males of Domestic Dog (

摘要

Canine reproductive behavior can be easily observed; however, the mechanism of semiochemical signaling in this species is still not well understood. Despite numerous studies, no efficient, artificial canine sex pheromones are available. In most studies of canine semiochemical communication, female urine was believed to be a source of volatile compounds that attract males. We hypothesized that urine is also a source of compounds that are very important in the process of the mating decision but are not so volatile. These compounds are collected by licking urine or the vulva and are transferred into the vomeronasal organ. Such behavior always precedes the male’s mating decision. In two experiments, we assessed the reactions of male dogs in response to air containing odor molecules from estrous females’ urine, from a live female in estrus, and from food, as well as during direct sniffing of urine samples from females in estrus, in anestrus, from male dogs and from humans. It was concluded that urine odor is not used for long-distance semiochemical communication in dogs but rather for close distance signaling.